Tuesday, October 13, 2009

Notes!

Blood

T. cruzi trypomastigote, epimastigote (is actually seen on a clear saline slide)

T. b. gambiense & rhodesiense- trypomastigote in blood epimastigote (saline slide because found in BUG)

Plasmodium falciparum- ring stage, troph, schizont, merozoate, gametocyte. Picture was of vessel near brain.

Babesia microti- merizoites in RBCs. Vector is tick.

Misc. Media (usually saline)

Leishmania- promastigote is in the vector

Trichomonas vaginalis (just a saline slide)- only has troph form.

Tissue

E. histolytica troph ( in any tissue)

Balantidium coli- trophozoite seen at 40x

T. cruzi- amastigote in heart tissue

Leishmania- amastigote in tissue (not in blood) in macrophages, in any visceral tissue but not in heart.

Toxoplasma gondii- tachyzoites (not in tissue cyst), merizoite?, Bradyzoites are seen inside the tissue cysts (i.e. schizonts) remember they look like the palmagranite!

Eimeria spp.- marizoites in Large intestine, megaschizont big guy

Cryptosporidium- paracytiferous vacuole in lining of intestines

Sarcocystis- muscle tissue seen at 10x

T. gondii- looks like guts with villi, oocytes, gametes seen in cat intestines.

Feces

Entamoeba coli cyst (4+ nuclei)

Trypanosoma cruzi- metacyclic trypomastigote (don’t need to identify as metacyclic just as a trypomastigote)

Entamoeba histolytica- troph and cyst seen

Chilomastix mesnili- cyst and troph seen

Trichomonas hominis- troph seen. Only a troph stage

Giardia lamblia- better seen on clear slides tho.

Iodamoeba- troph and cyst stages seen

Balantidum coli- cyst (only in feces), troph (seen in feces and tissue)

Isospera- sporulated/ unsporulated oocysts seen

Cryptosporidium parvum- little pink dots- are the oocysts in feces.

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